Use bd facscanto clinical software for performing cytometer quality control. Close buttonexits the application and prompts the fluidics shutdown procedure. Bd facscanto setup bd facsdiva setup entries in bolded text are setups created in bd facsdiva notice software. The bd facscanto comes with bd facscanto clinical software and bd facsdiva software. Performance of a novel bd stem cell enumeration kit on two. Minimize, maximize, and close buttons in title bar minimize buttonreduces the application to a button on the windows taskbar. Device modificationpae1 o56 bd facscanto system with bd facscanto clinical software pg 1o 5 01126105. Bd facs loader optional the bd facs loader automatically introduces prepared samples to the cytometer. The ivd cleared bd facscanto ii system includes software designed to address the. The bd facscanto ii prides itself on its ease of use and ability to provide proven performance, accuracy and highquality results. Bd biosceinces instrument and application trainings.
If you are not familiar with the windows operating system, refer to the documentation provided with your computer. Bd facscanto system a proven platform for high reliability and quality results a proven platform for high reliability and quality results built on more than 30 years of bd experience and leadership in flow cytometry and multicolor analysis, the bd facscanto flow cytometry system delivers reliable performance, accuracy, and easeofuse for todays busy clinical laboratories. Bd facscanto ii flow cytometer research brochure frequently asked questions. Bd facsdiva software provides one of the most complete and robust feature sets available for flow cytometry. Bd facscanto ii 422 and 53 configurations with bd facscanto clinical software and bd facsdiva software.
Bd facscanto clinical software with spa ii sample prepper bd facscanto clinical software with spa iii sample prepper using bd panels with bd facscanto clinical software and the bd facslink lis interface. Troubleshooting results with bd facscanto clinical software filter guide. There is enough amount of sheath fluid to perform machine start up and sample acquisition. It analyzes cells as they pass through a focused laser beam one at a time in a moving. When you install tubes onto the sit, a pump within the fluidics cart pressurizes the plenum, which then provides sheath fluid to the flow cell. The february 2020 security rollupcumulative update was released in february 2020. Labeling of whole blood with bd hlab27 reagents, manual preparation. These panels feature automated gating, calculations, and report generation to deliver consistent, reproducible, and standardized analysis.
Firsttime users of bd facsdiva software should read. Bd facs canto ii operators manual marshall scientific issuu. Bd facscanto ii resources cell analyzer research instrument. Performance of a novel bd stem cell enumeration kit on two flow cytometry systems. Win32k patch was released with december 2019 security updates, crypto api patch was released with january 2020 security updates. Page 1 bd facscanto ii flow cytometer quick reference guide this guide contains instructions for both bd facscanto clinical and bd facsdiva software. The bd facsdiva software reference manual assumes you have a working knowledge of basic microsoft windows operation. Vitro diagnostics assays including bd s tbnk analysis kits.
This software may not be duplicated, reproduced, or copied in any form or by any means whatsoever, except as otherwise permitted by law. For bd facsdiva software, run the bubble filter purge and degas flow cell command at least once you might need to run it several times. The spa 1i and manual pipetting are used for the same sample type and result in similar performance characteristics. Advancing clinical discovery research with our clinical discovery research reagents cdrr we want to help clinical researchers advance their clinical discovery.
Bd facscanto clinical software is supplied with the bd facscanto and facscanto ii systems and includes application panels optimized for use with specific ivd reagent kits. Bd facscanto ii flow cytometer and bd facscanto clinical software faqs. The end result is a patented optical design for enhanced signal retention coupled with digital electronics that capture up to 10,000 events per second. Reference manual, bd facscanto clinical software reference manual, or the bd facscanto ii instructions for use specific to the operation you are performing. Sit cleaning between tubes is automatic when you use bd f acscanto clinical. The bd facsverse system reference is a comprehensive collection of information that includes all content from this users guide and additional con cepts, procedures, and reference information about the cytometer and bd facsuite software. Developed in collaboration with data innovations, a leading middleware provider experienced in clinical applications, this lis interface solution allows the direct transfer of information between bd clinical instrument software and an lis, reducing errors commonly caused by manual transcriptions.
Bd facscanto clinical software 1 prepare a worklist. Pdf performance of a novel bd stem cell enumeration kit on. A bd facscan system the facscan system is an automated. The loader is controlled by bd facscanto clinical software or bd facsdiva software. The bd bioscience facscanto ii flow cytometer is a benchtop analyzer providing reliable and accurate results for experiments with up to 10 parameters. Top up the tubes from the wash bottles if required. Using bd panels with bd facs spa and bd facscanto clinical software and the bd facslink lis interface. The kit is intended for in vitro diagnostic use on either a bd facscalibur flow cytometer using bd cellquest v3. For equivalency between methods, data n 362 were analyzed with deming regression for absolute count and percentage of lymphocytes. For information on how to operate and maintain your flow cytometer, refer to the instrument users guide. Bd facscanto ii flow cytometer quick reference guide. The bd facslyric flow cytometer is for in vitro diagnostic use with bd facsuite clinical software for up to 6 colors. Instruments available for use at the flow cytometry core facility, institute of cell and molecular science, barts and the london, queen mary, university of london.
Bd facscanto ii flow cytometer and bd facscanto clinical software. The bd facscanto clinical software reference manual assumes you have a working knowledge of basic microsoft windows operation. Bd facscanto ii quick reference manual pdf download. For instructions on creating a user name and password, refer to the. Troubleshooting the tips in this section can help you troubleshoot issues that might arise when using this cytometer. Bd facscanto or bd facscanto ii flow cytometer and fluidics cart. Multisite evaluation of the bd stem cell enumeration sce. Bd facs canto ii flow cytometry system gmi trusted. The bd facslyric flow cytometer is for research use only with bd facsuite software for up to 12 colors. Bd facscanto clinical software contains modules for dedicated. At the same time, the sample tube is pressurized and sample is pushed up the sit and into the flow cell. Setup on page 1 in the bd facsdiva software reference manual. The tbnk kits are capable of providing a complete immune panel t.
The bd facsdiva software reference manual assumes that you have a working knowledge of basic microsoft windows operation. The bd cytometer setup and tracking application guide assumes that you have a working knowledge of basic microsoft windows operation. It provides a high degree of automation and quality control to reduce hands on time for technicians and improve reliability of results. Bd hts option on bd facscanto instruments, new look and feel, ability to disable biexponential scaling, apply scaling values to other elements in experiments, scale to population, copypaste. Biexponential display the biexponential display feature has been enhanced as follows. Ed facscanto system with bd facscanto clinical software using manual pipetting.
For software troubleshooting, refer to the bd facscanto clinical software reference manual, the bd facsdiva software reference manual, or the bd facscanto instructions for use. In bd f acsdiva software, sit cleaning between tubes is automatic. Each sale of a stored unit of this software grants the purchaser a nontransferable, nonexclusive, personal license. View online quick reference manual for bd facscanto ii measuring instruments or simply click download button to examine the bd facscanto ii guidelines offline on your desktop or laptop computer. The modules feature automated gating, calculations, and report generation to deliver a consistent, reproducible, and standardized analysis. The bd facscanto ii instructions for use assumes you have a. If you are not familiar with the windows operating system, see the documentation provided with your computer. Download user manual manual of bd facscanto ii laboratory equipment, measuring instruments for free or view it online on. It provides a high degree of automation and quality control to reduce hands. Standardization of 8color flow cytometry across different. Course prerequisites basic computer skills are required for the course duration. The bd facscanto ii is a reliable benchtop analyzer that has stemmed from more than 20 years of pioneering innovation in flow cytometry and multicolor analysis. Bd facscanto ii flow cytometer quick reference guide this guide contains instructions for both bd facscanto clinical and bd facsdiva software.
Bd facscanto ii canto 10color with canto clinical software. Additionally, when used with bd trucount standard tubes, the. Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. All other cytometer and fluidics cart functions are controlled by bd facscanto clinical software and bd facsdiva software provided with the instrument. The facscanto ii offers unbeatable consistency and optimal signal detection with increased resolution and sensitivity for multicolor assays. Bd facscanto ii flow cytometer reference manual alfresco. Bd facscanto ii measuring instruments user manual manual pdf. Our recently expanded portfolio of antibodies and bright fluorescent dyes enables bd to offer a broad selection of kits to advance your clinical research needs. Bd facscanto ii operation manual 1 facscanto ii standard operation protocol basic operation 1. Click reference to open the bd facsverse system reference in a web browser. Our recently expanded portfolio of antibodies and bright fluorescent dyes enables bd to offer a broad selection of kits to advance your clinical. Bd facsdiva software reference manual for version 8.
Bd facscanto clinical software includes specific application modules optimized for use with specific ivd reagent kits. Calculate compensation, and name the resulting setup. Computer workstation runs two software packages facscanto clinical software for automated immunophenotyping and bd facsdiva software for manual immunophenotyping of laboratory developed tests ldt. Bd facscanto ii measuring instruments quick reference manual. Before using this manual, make sure you are familiar with general cytometer functions, as described in the cytometer manual. About this manual this manual describes how to use bd facsdiva software. The instrument can simultaneously measure forward scatter, side scatter. This guide should help you operating the flow cytometer and leaving it such way that another colleague can use it another day. Make sure the following steps are done before running your samples. Bd facs canto ii flow cytometer quick user guide v.
Productivity, consistency, and reliability from dedicated clinical software. Bd biosciences facscanto clinical software features. The bd facscanto ii is designed to address the needs of todays busy clinical lab. Bd facscanto ii measuring instruments user manual manual. Waste tank is empty and refill the bottom layer of waste tank with bleach. Maximize buttonfills the screen with the main window. Follow only those sections that apply to the software you are running. This software is the property of becton, dickinson and company. Bd facscanto ii 422 configuration automated sample presentation bd facs universal loader bd facsloader with updated motor, pneumatic actuation, and sliding access doors software bd facsuite clinical software with 4 and 6color assay modules facscanto clinical software with multitest 6color and multitest 4color assays included as panels. Facscanto ii standard operation protocol basic operation. Biexponential display is enabled by default, but can be disabled in user preferences, allowing more events to be recorded per experiment. View online user manual manual for bd facscanto ii measuring instruments or simply click download button to examine the bd facscanto ii guidelines offline on your desktop or laptop computer. Running samples with bd facscanto clinical software 89running an.
Bd facscanto clinical software contains modules for dedicated clinical applications with automatic gating algorithms, while bd facsdiva software is non. Bd facscanto fluidics with bd facscanto clinical software or bd facsdiva software. The bd facscanto clinical software reference manual assumes you have a. Samples were analyzed on the bd facslyric system with bd facsuite clinical software, and on the bd facscanto ii system with bd facscanto clinical software and bd facs 7color setup beads.
This guide contains instructions for both bd facscanto clinical and. System options include 8color 3laser system 405 488 633 nm 8color 2laser system 488 633 nm a 2d barcode reader option a choice of two automated sample loading systems with integrated covers. See headers and footers on page 100 in the bd facsdiva software reference manual. Bd biosciences recommends that firsttime users of this cytometer take advantage of operator training offered with the. For bd facscanto clinical software, run the bubble filter purge command at least once you might need to run it several times. Saves time, improves reproducibility high performance, innovative system the bd facscanto ii fluidics cart is designed to save time and improve system performance. This guide contains instructions for using bd facscanto clinical software with bd facscanto and bd facscanto ii systems with the bd facs loader.
Multisite evaluation of the bd stem cell enumeration kit for. Before you begin this guide assumes that you have read the bd facscanto ii instructions for use and the bd facscanto clinical software reference manual, and that you are familiar with cytometer setup. The bd facscanto clinical software is used for fda. Install the first sample tube manual acquisition or the loader rack automated acquisition, and click when asked if you want to save the worklist, click no. Standardization of 8color flow cytometry across different flow cytometer instruments.
Accurate and reproducible enumeration of t, b, and nk. System options include an automated facs universal loader and a. Bd bioscience facscanto ii flow cytometer marshall. Bd facscanto flow cytometer reference manualk pdf4pro. Download quick reference manual of bd facscanto ii laboratory equipment, measuring instruments for free or view it online on. Becton dickinson bd facscanto ii and beckman coulter bc navios, aiming at increasing reproducibility and interlaboratory comparability of immunophenotypic data in clinical laboratories in. Bd facscanto clinical software generated a lyseno wash and a lyse wash setup. Sections are indicated with the appropriate software icons. Fluidics cart contains operational fluids facsflow cubitainer, facsclean solution, facs shutdown solution, and waste container. Z bd facscanto ii clinical or facsdiva p d u v y z. Bd facscanto ii system 42 configuration with bd facscanto clinical software and bd facsdiva software, k062087 was also used a reference device. Refer to the bd facsdiva software reference manual for details.
Applying the setup results rightclick the experimentlevel cytometer settings, and choose apply setup. How to operate the bd facscanto flow cytometer preface dear colleague, the bd facscanto flow cytometer is use to operate, however, it is a delicate instrument for measuring fluorescence of single cells. Bd bioscience facscanto ii flow cytometer marshall scientific. A feasibility study in clinical laboratories in switzerland. Reap the benefits of supreme flexibility by configuring read more. Bd facs canto ii operators manual marshall scientific. New worksheet features create headers and footers on the worksheet, including cytometer name, experiment name, user name, date and time, bd facsdiva software version, etc. Multisite evaluation of the bd stem cell enumeration kit.
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